Safe inoculation of blood for liquid culture
نویسندگان
چکیده
منابع مشابه
Direct inoculation procedure for the rapid classification of bacteria from blood culture.
A procedure was developed for 4-h identification of bacteria from blood culture. From a turbid blood culture bottle a 10- to 15-ml aliquot was drawn off and centrifuged. The pellet was utilized to inoculate a series of enzymatic and physiologic reactions. Three hundred eight positive blood cultures were tested including 222 strains of Enterobacteriaceae, 40 strains of facultative grampositive c...
متن کاملMyeloid differentiation of human blood mononuclear cells in liquid culture.
Human blood mononuclear cells were prepared from peripheral blood or single donor plateletpheresis residues by depletion of adherent cells and T lymphocytes. In double-layer soft agar culture, 5 x 10(5) such cells yielded from 33 to 165 colonies. For liquid culture, these cells were suspended in McCoy's 5A medium with 15% fetal bovine serum and 0%, 20%, or 40% conditioned medium (CM) and incuba...
متن کاملImproved diagnosis of orthopedic implant-associated infection by inoculation of sonication fluid into blood culture bottles.
Sonication improved the diagnosis of orthopedic implant-associated infections (OIAI). We investigated the diagnostic performance of sonication fluid inoculated into blood culture bottles in comparison with that of intraoperative tissue and sonication fluid cultures. Consecutive patients with removed orthopedic hardware were prospectively included and classified as having OIAI or aseptic failure...
متن کاملA Simple Apparatus for the Automatic Inoculation of Culture Media †
In connection with certain investigations being carried out in this laboratory, it was found necessary to work with sixto twelve-hour cultures of bacteria in fluid culture media. The apparatus described below was devised to obviate the need of either coming to the laboratory late in the evening to inoculate cultures for the following day's work or of wasting time waiting for cultures to grow ou...
متن کاملBedside inoculation of blood culture bottles with ascitic fluid is superior to delayed inoculation in the detection of spontaneous bacterial peritonitis.
Ascitic fluids from patients suspected of having spontaneous bacterial peritonitis were inoculated into blood culture bottles (i) at the bedside and (ii) in the laboratory after a delay. In 29 episodes in which the bedside bottles were culture positive, only 22 (75.9%) of the laboratory-inoculated sets demonstrated growth; this difference was statistically significant (P less than 0.02).
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ژورنال
عنوان ژورنال: Occupational Medicine
سال: 2005
ISSN: 1471-8405,0962-7480
DOI: 10.1093/occmed/kqi103